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PRE-SOLICITATION NON-COMPETITIVE – Notice of Intent to Sole Source SOLICITATION NUMBER: PCA-NIA-02902 TITLE: Single-cell RNA sequencing reagents RESPONSE DATE: April 17, 2026, at 4:00 pm EST. PRIMARY POINT OF CONTACT: Jaddua Johnston Jaddua.Johnston@nih.gov Phone: 240-569-0006 INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT WITHOUT PROVIDING FOR FULL AND OPEN COMPETITION (INCLUDING BRAND-NAME). The Antibody Diversity Section at the National Institute on Aging (NIA) is investigating the hypothesis that patients with Alzheimer's disease exhibit increased immune cell infiltration into the cerebrospinal fluid (CSF). To support this research, the laboratory will perform single-cell RNA sequencing (scRNA-seq) on cellular pellets from approximately 60 human CSF samples. This requirement is for reagents necessary to partition cells into single-cell Gel Beads-in-Emulsion (GEMs) and generate barcoded cDNA libraries for downstream RNA sequencing analysis. These reagents will enable identification of immune cell populations and characterization of transcriptional differences between healthy and disease states. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE and SET ASIDE STATUS The North American Industry Classification System (NAICS) code for this requirement is 334516 – Analytical Laboratory Instrument Manufacturing , with a size standard of 1,000 employees. This acquisition is not set aside for small business . REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular (FAC) 2024-07, dated August 29, 2024. This acquisition is conducted under the procedures prescribed in FAR Part 12 – Acquisition of Commercial Products and Commercial Services and FAR Subpart 13.5 – Simplified Procedures for Certain Commercial Products and Commercial Services . STATUTORY AUTHORITY This acquisition is conducted under the authority of FAR 6.302-1 – Only One Responsible Source and No Other Supplies or Services Will Satisfy Agency Requirements (41 U.S.C. 3304(a)(1)) . DESCRIPTION OF REQUIREMENT: Purchase Description Single-cell RNA sequencing reagents compatible with the 10x Genomics Chromium platform, including: Library Construction Kit, 16 reactions (Part No. 1000190) GEM-X Universal 5' Gene Expression v3, 16 samples Associated shipping and handling Salient characteristics : The required reagents must meet the following minimum technical characteristics: Compatibility with the 10x Genomics Chromium Controller system currently in use at NIA Capability to partition individual cells into Gel Beads-in-Emulsion (GEMs) Support for 5' gene expression profiling workflows Integrated single-cell barcoding for downstream RNA sequencing Validated performance for human-derived biological samples, including CSF Ability to generate high-quality cDNA libraries suitable for next-generation sequencing Quantity: Quantity 2: Library Cons…
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