The National Institutes of Health (NIH), Office of the Director (OD) is conducting a market survey to determine the availability and technical capability of qualified small businesses, including 8(a) small businesses, small, disadvantaged businesses, woman-owned small businesses, servicedisabled veteran-owned small businesses or HUBZone small businesses capable of providing a new Brand name or equal to Leica Microsystems Stellaris Microscope System with Confocal, 3DSTED, FLIM, and Spectral Multiplexing imaging capabilities. An NIH lab requires acquisition of a Leica Stellaris microscope system to provide investigators with substantially improved sensitivity, resolution, and advanced imaging modalities needed for modern cellular and subcellular research. The current Leica SP8 confocal microscope has served as an imaging workhorse for more than a decade, supporting approximately 25 laboratories in the past year and an estimated 50 laboratories over its lifetime. The SP8 will be traded in as a line-item discount toward the new system. The Stellaris platform is required to enable advanced confocal imaging, three-dimensional STED super-resolution, fluorescence lifetime imaging (FLIM), and high-plex spectral multiplexing workflows (up to 15 colors) to support spatial-omics–type studies and other complex multi-parameter imaging experiments. Product Specifications/Features/Salient Characteristics Required Minimum Specifications: · Confocal excitation: white light laser with tunable excitation range 440–790 nm with AOBS system. · Prism-based fluorescence detection: prism-based spectral detection with variable emission windows to 850 nm. · Detectors: five (5) Hybrid (HyD) detectors optimized for spectral detection across approximately 400–850 nm. · 3D STED super-resolution: pulsed depletion at 775 nm and 589 nm, with threedimensional STED capability. · FLIM: integrated fluorescence lifetime imaging module for lifetime measurements, enhancement of super-resolution performance, and lifetime-based fluorescence species separation. · Pulse picker: capability to reduce/adjust the white light laser repetition rate to optimize FLIM and related applications. · Scanning modes: ability to fast-switch between galvo and resonant scanning to support both high-resolution and high-speed imaging needs. · Objective correction-collar support (existing lenses): motorized correction collar hardware and software support for existing Leica 100x oil and 93x glycerol objectives. · New objective: a Leica 86x water-immersion objective with correction collar, chromatically corrected across the full 440–790 nm excitation range and optimized for 589 nm and 775 nm STED depletion, to ensure accurate multi-color registration and optimal super-resolution performance. · High-plex spectral multiplexing: capability to image of 15 (or more) fluorescent colors per sample, including tools to plan spectral multiplexing panels and perform spectral unmixing. TRADE- IN EQUIPMENT: The lab/government …