The purpose of this amendment is to provide Questions and Answers: Question 1: Could the PI share the target region info or reference article so we can take a look? If default primers like 16S, ITS, or 18S are acceptable, that's fine; otherwise, we will need the PCR cycle conditions. Answer 1: The assay is not published yet and so I can¿t share the details until the vendor is selected. Question 2: The RFQ lists both CLIN 00010 and CLIN 00040 as ¿Metabarcoding Analyses¿ in accordance with Attachment 1. Can you clarify the intended distinction between CLIN 00010 and CLIN 00040, including whether they should be priced separately, whether they represent different assay/sample types, or whether one is an option/additional quantity? Answer 2: CLIN 00010 and 00040 are for the same type of analyses, just for different funding accounts. The U.S. Geological Survey, Conservation Genetics Lab, Wetlands and Aquatic Research Center in Gainesville, FL, requires genomic services of molecular library preparations and next generation sequencing with specialized bioinformatics as described in Attachment 1 statement of work. The firm, fixed-price contract is for a one-year period. Quotes shall provide pricing, and technical capabilities and experience to perform the work. The basis of award is lowest-price technically acceptable (see E.9. Evaluation). Quote submissions are due on or before 7/02/2026. The anticipated award date is 7/16/2026. The acquisition is not a small business set-aside. The NAICS is 541380. Vendors must be registered at https://www.sam.gov/ to be awarded the contract. All responsible sources may submit a quotation which shall be considered by the government. All correspondence and quotes MUST be emailed to sruggles@usgs.gov.