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The National Institutes of Health (NIH), Office of the Director (OD) is conducting a market survey to determine the availability and technical capability of qualified small businesses, including 8(a) small businesses, small, disadvantaged businesses, woman-owned small businesses, servicedisabled veteran-owned small businesses or HUBZone small businesses capable of providing a new Brand name or equal to Cytek Biosciences: Cytek Aurora EVO 5 Laser UV/YG/V/B/R and Cytek Aurora-CS 5 Laser UV/YG/V/B/R. Product Specifications/Features/Salient Characteristics Required Minimum Specifications: The request is for a 5-laser spectral flow cytometry system consisting of both a spectral analyzer and a 6-way spectral cell sorter with small particle detection, designed for high-plex and small particle studies. Core System Requirements · Both analyzer and sorter must: · Use software platform to enable seamless experiment/data transfer and analysis. · Support spectral continuity between analyzer and sorter. · Operate with a 5-laser configuration: o UV (355 nm) o Violet (405 nm) o Blue (488 nm) o Yellow-green (561 nm) o Red (640 nm) · Detect fluorescence up to 825 nm or higher wavelength. · Perform live spectral unmixing. · Calculate autofluorescence as a separate parameter. · Generate data compliant with Flow Cytometry Standard (FCS) 3.1 or higher. · Be compatible with major analysis platforms (OMIQ, FlowJo, Cytobank, FCS Express, etc.). · Include an automated QC module. · Include all required attachments for full functionality. Analyzer-Specific Requirements · Equipped with an autoloader supporting: o 96-well standard plates o 96-well deep-well plates o 384-well plates o Individual or rack-based 12 × 75 mm tubes · Protocols developed on the analyzer must be transferable to the sorter. Sorter-Specific Requirements · 6-way sorting capability. · Capable of aseptic sorting into: o 1.5–1.8 mL Eppendorf tubes o 96-well cell culture plates o 384-well plates o 96-well PCR plates · Support for 70 μm, 100 μm, and 130 μm nozzle sizes. · Include a vendor-tested biosafety hood for proper aerosol containment. · Must support small particle detection. Overall, the system must provide integrated, high-plex spectral particle/cytometric analysis and high-plex spectral cell/particle sorting with seamless workflow continuity, advanced unmixing capabilities, small particle detection, and compliance with current data standards. Both units must meet NIH workplace instrumentation requirements. Instruments must come with 5-year extended warranty and onsite field service support. Interested firms with the capability of providing all the required items shall submit a capability statement to assist the Government in determining in accordance with the Federal Acquisition Regulation 19.104-1. The intended procurement will be classified under North American Industrial Classification (NAICS) code 334516 with a size standard of 1000e. All respondents are required to identify their …
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